Live mammalian cell arrays
نویسندگان
چکیده
منابع مشابه
Integration column: microwell arrays for mammalian cell culture.
Microwell arrays have emerged as robust and versatile alternatives to conventional mammalian cell culture substrates. Using standard microfabrication processes, biomaterials surfaces can be topographically patterned to comprise high-density arrays of micron-sized cavities with desirable geometry. Hundreds to thousands of individual cells or cell colonies with controlled size and shape can be tr...
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Single-step methods for the generation of patterned surfaces on hydrogels are presented. Poly(vinyl alcohol) films covalently bonded on glass cover slips and commercially available hydrogel-coated polystyrene plates were used as cell-repellent surfaces. Cell-adhesive domains were created by spotting dilute solutions of sodium hypochlorite onto the surfaces. Alternatively, domains supporting cel...
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We investigated the molecular determinants of aquaporin-4 (AQP4) assembly in orthogonal arrays of particles (OAPs) by visualizing fluorescently labeled AQP4 mutants in cell membranes using quantum-dot single-particle tracking and total internal reflection fluorescence microscopy. The full-length ;long' (M1) form of AQP4 diffused freely in membranes and did not form OAPs, whereas the ;short' (M2...
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Genetic code expansion and bioorthogonal labeling provide for the first time a way for direct, site-specific labeling of proteins with fluorescent-dyes in live cells. Although the small size and superb photophysical parameters of fluorescent-dyes offer unique advantages for high-resolution microscopy, this approach has yet to be embraced as a tool in live cell imaging. Here we evaluated the fea...
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Recent reports suggest that extranuclear targets in cytoplasm may have a role in mediating radiation effects in mammalian cells exposed to ionizing radiation. We have focused mitochondria as a target of ionizing radiation, particularly heavy ions, because mitochondria are a kind of organelles existing widely in cytoplasm. They play a vitally important role of ATP formation through the operation...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2013
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.2473